Figure 7.

Foci formation in OPTN_WT- and OPTN_E50K-overexpressing cells. TM cells were transiently transfected with pEGFP-N1, pOPTN_WT-GFP, or pOPTN_E50K-GFP for 16 hours. Images showing the transgene expression or foci formation (green) are presented in A. The images were taken using a 63× oil objective. Bar = 10 μm. Similar results were also obtained with RPE cells (data not shown). The number of foci in at least 60 transfectants was counted, and the average number of foci per transfectant is shown in B. Data presented were mean ± SD from three independent experiments.