Figure 3.

Knockdown of SDF-1 and decreased AFP expression after siSDF treatment. SDF-1 and AFP protein level in rat liver was detected by Western blot. Protein sample (10 μg) from the liver of each animal was loaded into each lane. Relative quantity of each band is determined by normalizing its total density to that of loading control in the same lane. Ad-siSDF-induced RNA interference results in considerable decrease in SDF-1 expression, accompanied by a decrease of AFP expression from these rat livers (P < 0.05, Student’s t-test).