Table 2.
Effects of stimuli on the filamentous morphology
| Stimulus added* | % yf† | % ff‡ | yf radius§ |
|---|---|---|---|
| Glu (1 M) | >95 | <5 | 20 |
| Glu (0.25 M) | >95 | <5 | 15 |
| Glu (0.06 M) | >95 | <5 | 4 |
| Sucrose (1 M) | >95 | <5 | 12 |
| Mannose (1 M) | >95 | <5 | 11 |
| Glycogen (100%) | <5 | >95 | 0 |
| Glycerol (100%) | <5 | >95 | 0 |
| Glu (1 M) + 0.1% SDS | >95 | <5 | 20 |
| Glu (1 M) + 1 M NaCl¶ | >95 | <5 | 20 |
| YPD | >95 | <5 | 20 |
| YP | <5 | >95 | 0 |
| Peptone (10%) | <5 | >95 | 0 |
| Yeast extract (10%) | <5 | >95 | 0 |
| Stimulus removed‖ | |||
| Nitrogen | 90 | 10 | NA |
| Amino acids | >95 | <5 | NA |
| Uracil | >95 | <5 | NA |
*
Stimulus (10 μl) was spotted onto discs and placed on SC medium; Glu, glucose.
†
% yf denotes round cells and axial budding for >200 microcolonies adjacent to the disc.
‡
% ff denotes elongated cells and unipolar budding for >200 microcolonies adjacent to the disc.
§
Distance (in mm) from the center of a disc to the point at which only ff colonies were observed.
¶
Some unipolar budding (<20%) was observed.
‖
SCD medium lacking the noted ingredient; for nitrogen, SLAHD medium was prepared as described in Materials and Methods; NA, not applicable.