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Bud site selection is influenced by glucose
| Cell | Bud | Bud pattern, %*
|
||
|---|---|---|---|---|
| Axial | Random | Opposite | ||
| M† | 2nd, 3rd, or 4th | 75 | 5 | 20 |
| D1 | 1st | 15 | 0 | 85 |
| D1 | 2nd | 98 | 0 | 2 |
| D2 | 1st | 13 | 0 | 87 |
| D2 | 2nd | 98 | 0 | 2 |
Cells were grown for 24 h on SC medium, scraped from the plates, and stained with Calcofluor as described in Materials and Methods. Cells on SCD medium were observed to have >98% axial budding.
For cells chosen, M, mother; D1, 1st daughter; D2, 2nd daughter.
