Skip to main content
. 2007 Jan;170(1):152–159. doi: 10.2353/ajpath.2007.060722

Figure 3.

Figure 3

Mutation analysis of transcription factor binding sites of the VCP promoter. The pGL3 basic plasmid containing 1.5-kb sequence of 5′-flanking region of the VCP gene was used as the parental clone.1 Plasmids containing mutation at −1132 and both of −113 and −1843 were sequentially generated and transfected to MCF7 (a), PC3 (b), and SW480 (c) cells. The luciferase activity showed a significant decrease with the transfection of the plasmid mutated at −113 and was completely lost with the transfection of the plasmid mutated at both −113 and −184. Bars represent mean ± SE of at least three independent experiments. *, **Significant difference (P < 0.05, P < 0.001, respectively) compared with the parental construct. Luc, luciferase.