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. 2007 Jan;170(1):20–32. doi: 10.2353/ajpath.2007.060452

Figure 2.

Figure 2

Regulation of MUC5B secretion by PMA. Cells were treated with or without 10 nmol/L PMA. Cell lysates were harvested and prepared at 8, 16, and 24 hours after treatment. MUC5B protein was measured by ELISA as described in Materials and Methods. The quantity of MUC5B protein was presented as picomoles of MUC5B-equivalent/milligram of total protein, using the synthetic 19-amino acid peptide immunogen as the reference. Triplicate dishes were used for each protein ELISA, and experiments were repeated at least three times in different cultures derived from different donors and passages of cell lines, with similar results. Significance: *P < 0.05 and **P < 0.01, respectively, compared with unstimulated control at each time point. A: NHBE cells. B: A549 cells. C: HBE1 cells.