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Turnover of WT and mutant MYOC-GFP is not influenced by proteasome inhibition. Autoradiogram of metabolically labeled and immunoprecipitated WT, P370L, and Y437H MYOC chased in the presence of 10 μmol/L MG132. Identical results were obtained for HTM cells (shown here) as well as HEK293 and CHO cells (not shown). Western blotting of GAPDH in soluble cell lysates served as loading control.
