Figure 6.

Heteromeric mutant/WT MYOC aggregates induce apoptosis. A: HEK293 cells stably expressing only C245Y MYOC-GFP appear in green whereas those co-expressing the mutant and the FLAG-WT MYOC can be identified by their orange-yellow color. B: The some cluster of cells as shown in A but stained by Hoechst 332518 DNA stain. The C245Y MYOC-GFP and FLAG-WT MYOC co-expressing cells exhibit fragmented nuclei C: Apoptosis rate was highest in C245Y/WT-expressing cells when compared with WT, WT/WT, or C245Y MYOC-expressing cells and increased with time after transfection. D: Apoptosis rate of different mutant/WT MYOC-expressing cells at day 5 after pFLAG-MYOC^WT transfection.