Figure 4.

The role of IκBα in IFNα/β promoted cell survival. IFN-treated (1,000 units/ml; 24 h) Daudi cells transiently transfected for 48 h with IκBαM, IκBαΔN, or empty vector were analyzed for apoptosis by TUNEL assays (A and B) or apoptotic DNA by a chemiluminescent assay with a DNA ladder provided for reference (C). Apoptotic cells are fluorescent and thus appear green in the photomicrograph. The TUNEL data (B) represent the mean of three independent experiments in which at least 500 cells were scored for each variable (SEM < 10%).