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. 2000 Nov 28;97(25):13631–13636. doi: 10.1073/pnas.250477397

Figure 5.

Figure 5

IFN induces NF-κB activation in diverse cell types. (A) EMSA on nuclear extracts from IFN-treated (5,000 units/ml rat IFNβ for 30 min) murine 3T3 fibroblasts and rat intestinal IEC-6 cells, or IFN-treated (5,000 units/ml IFNCon1 for 30 min) human CaKi and HepG2 cells. (B) Murine L929 cell transfectants expressing empty vector (EV) or two independent wild-type huIFNAR-1 (WT) transfectants (29) were treated with IFNCon1 (5,000 units/ml) for 30 min before gel shift analysis with a NF-κB probe.