Figure 4.

The kinetics of anti-CC2 and anti-CC2 peptide antibodies in CC2-immunized (A) and CC2P12-immunized (B) rats. A: The levels of anti-CC2 and anti-CC2 peptide antibodies were determined using ELISA. Recombinant CC2 and CC2P1-P12 (10 μg/ml) were coated onto microtiter plates, and diluted sera from CC2-immunized rats were applied. After washing, horseradish peroxidase-conjugated anti-rat IgG was allowed to react. The reaction products were then visualized after incubation with the substrate. The absorbance was read at 450 nm. B: The kinetics of anti-CC2 and anti-CC2 peptide antibodies in CC2P12-immunized rats. During the observation period, only anti-CC2P12 and anti-CC2 antibodies were elevated.