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Van1p-myc, Anp1-myc, and Mnn9p-myc are enriched in COPII vesicles generated from microsomes of cycloheximide-treated cells. Vesicles (right two lanes) were generated by incubation of microsomal membranes with purified COPII components, and proteins were resolved on SDS/PAGE. For quantitation, 30%, 20%, 10%, 5%, and 1% fractions from the total membrane content were compared with vesicle proteins by immunoblot (left five lanes).
