Table 1.

Spindle Parameters

Mutation in DAM1a	Total number of Spindlesb	Mean Spindle Length (μm)c	Number of Metaphase Spindlesd	Mean Metaphase Spindle Length (μm)e	Mean Distance between Kinetochores (μm)f
WT	175	1.19 ± 0.33	115	1.25 ± 0.17	0.74 ± 0.24
S217A	116	1.17 ± 0.33	72	1.28 ± 0.20	0.79 ± 0.20
S218A	193	0.98 ± 0.38	100	1.11 ± 0.20	0.90 ± 0.19
S221A	119	1.09 ± 0.39	55	1.23 ± 0.18	0.96 ± 0.18
S221F	218	1.03 ± 0.41	108	1.11 ± 0.19	0.90 ± 0.19
S218A S221A	200	0.92 ± 0.39	81	1.11 ± 0.22	0.90 ± 0.21

^aStrains are given in Table S5, lines 33–38.

^bTotal number of budded cells with spindles entirely within the mother cell.

^cThe mean distance between SPBs labeled with Spc97-CFP in cells with both SPBs in the mother cell. Distance is shown ± the standard deviation.

^dMetaphase spindles were chosen as described in the Experimental Procedures.

^eMean distance between SPBs labeled with Spc97-CFP. Only metaphase spindles with clustered kinetochores were measured. Distance is shown ± the standard deviation.

^fMean distance between kinetochore clusters marked with Nuf2-Venus. Only metaphase spindles were measured. Distance is shown ± the standard deviation.