Table 3.
Primer pairs used to amplify by RT-PCR protostome and tunicate family 2 GPCRs
| Primer pairs | |
| Nematode | CelC13B9.4F1 5' -gatacacgaatttggtgtaatgcc-3'/CelC13B9.4R1 5' -gttcgtgtgaggaccattttcac-3' |
| C. elegans (Cel) | CelC18B12.2F1 5' -ccattcacattttgcactgcaatt-3'/CelC18B12.2R1 5 -gaaccagagaatagctttgcaaat-3' |
| Insect | DmeCG13758F 5' -gagattatccgtctcatgca-3'/DmeCG13758R 5' -cgcgttcaacgtggccgtt-3' |
| D. melanogaste (Dme) | DmeCG8422F 5' -agctgcccaccattatctac-3'/DmeCG8422R 5' -gttctggttaagctgaatggt-3' |
| A. gambiae (Aga) | DmeCG32843F 5' -gcatcacgctgcacatgaat-3'/DmeCG32843R 5' -cgccgagatgatttcgtatg-3' |
| AgaP14164F 5' -agcttcgagccggaaattgag-3'/AgaP14164R 5' -ttcgtgatcagcacccacatgat-3' | |
| Tunicate | CinS93F 5'-taccgcttggcgatttctg-3'/CinS93R 5' -aacgacatagagtagcaacga-3' |
| C. intestinalis (Cin) | CinS372F 5'-aacgacatagagtagcaacga-3'/CinS372R 5'-cggacgaaaatcaaactatg-3' |
| CinS752F 5'-attgctcacgtgacggtaga-3'/CinS752R 5'-ctccacaaatatttcttgtc-3' | |
| CinS273F 5' -gttttagaaaccggaacatg-3'/CinS273R 5' -agaaaaactgtttcgccggt-3' | |
| Cin50F 5'-gatccataggaagttgaaaag-3'/CinS50R 5' -aatgaaaataatttctccggtt-3' | |
| CinS70F 5'-cagctgtcgactagtaataac-3'/CinS70R 5' -gtatacagagacgatttccttg-3' | |
| CinS5AF 5'-cctggtatttttgatggttat-3'/CinS5AR 5'-attaggtatcatattgtttac-3' | |
| CinS5BF 5' -cctggtttctttgatagacaa-3'/CinS5BR 5' -agttcgtaaagcgttgtagat-3' | |
| Ciona 18S control | Cin18SFwd 5'-cggagaagtttcagcaca-3'/Cin18SRev 5'- agtgtcgcaaacccctgt-3' |
| 18S control | Sb18SFwd 5'-tcaagaacgaaagtcggagg-3'/Sb18SRev 5'-ggacatctaagggcatcaca-3' |
| β-actin control | SbDebactF3 5'-ggccgcgacctcacagactac-3'/SbDebactR2 5'-accgaggaaggatggctggaa-3' |