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ELISA values for positive reference serum and soluble E. cuniculi antigen diluted 2-fold from 1:1000. Each line represents the following coating buffers: -■- carbonate bicarbonate (100 mM, pH 9.6) -▲- citrate phosphate (70 mM, pH 4.0) -◆- distilled water -●- phosphate buffered saline (PBS; 170 mM, pH 7.4) -▼- citrate phosphate (150 mM, pH 5.0) -□- ethanol (70% v/w).
