Figure 1.
A mutant aptamer with enhanced affinity for its target. (A) Interaction mating assay between LexA-Cdk2 and aptamer 10, two strains carrying aptamer 10M, and a noninteracting aptamer C4, using three different sensitivity LexAop-lacZ reporters. (B) Western blot assay using an anti-TrxA antibody. Diploid exconjugates were grown in galactose-containing medium, and proteins were extracted and subjected to a Western blot analysis with anti-TrxA antibody. (C) Strength of interaction phenotypes as determined by fluorescence from a LexAop-GFP reporter plotted against Kd values measured in evanescent wave experiments. Fluorescence values are in arbitrary units (a.u.). (D) Sequence of the variable regions of aptamers 10 and 10M.