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Mapping sites on Cdk2 bound by the original aptamers and by aptamer 10M. We collected the Cdk2 mutant bait proteins, described elsewhere (3), Cdk3, and Drosophila Cdc2 and Cdc2c (16). In this experiment, we expressed TrxA, the 14 different aptamers originally selected (1), and aptamer 10M as preys. We mated yeast to generate an interaction matrix (16).
