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. Author manuscript; available in PMC: 2008 Jan 2.
Published in final edited form as: Mol Cell Endocrinol. 2006 Nov 9;260-262:49–58. doi: 10.1016/j.mce.2006.01.017

Figure 4.

Figure 4

Evolutionary conserved regions in the Fshr locus have associated transcriptional activity. (A) The human, rat and chicken Fshr loci between Nrxn1 and Lhcgr were analyzed for evolutionary conservation using the web-based genome navigation tool ECR browser (http://ecrbrowser.dcode.org/; Ovcharenko et al., 2004). Seven evolutionarily conserved regions (ECRs) were identified and annotated below the sequence (dark peaks marked 1-7). Each ECR was cloned in front of the minimal Fshr (-220/+123) Luc promoter and transfected together with phRL-TK into (B) primary rat Sertoli cells and (C) peritubular myoid cells, a non-expressing cell type. The data are represented as the firefly/Renilla luciferase activity ratio of each ECR-containing construct relative to the firefly/Renilla luciferase activity ratio of Fshr (-220/+123) Luc. Transfections were performed a minimum of three times. Error bars represent the standard errors of the means. Statistically significant differences (p≤0.002) in activity of ECR-containing promoters relative to that of the basal promoter (Fshr - 220bp/+123bp) are noted by “a” above the bars. Statistical significance was determined by a student’s T-Test.