Table 2.

Evolutionarily conserved regions (ECRs) in the human, rat and chicken Fshr loci.

	Previous designation*	% Identity#	Position (kb)§	Size (bp)	Cloning primers†
ECR1	site110	89.4%	-81	501	5’-GTACACACCTCTTTCTGCCTGGG-3’ 5’-GGTGGGGGCTCAGTCTGCTG-3’
ECR2	site160	85.6%	+401	543	5’-GGTCACCGAACACAGAATAATG-3’ 5’-GGAGGTGCCCATATTTTTGA-3’
ECR3	site161	84.4%	+429	284	5’-CTAATACATCCACAGCAGCCC-3’ 5’-CAATCCACAGATACTTTCGCAC-3’
ECR4	site73	83.3%	-265	202	5’-GGTGAACACTGGTCTTATTTGTC-3’ 5’-GTTTACGGTTGTGAAGGAGAGG-3’
ECR5	site63	83.3%	-334	153	5’-CTAAAAATGTTCCACGAGGTAGG-3’ 5’-CTTTTAAGTCCTGTTTTAAATCTTC -3’
ECR6	site38	81.8%	-474	294	5’-CACCCACAGATGCTTTCCTAC-3’ 5’-GTAAAACTAACTAGCACATAGCC-3’
ECR7	site34	83.0%	-489	316	5’-CTTTGCCTATAAATAAGATGTCAAC-3’ 5’-GGTGATTTCTAATCTCATTATACCC -3’

^*As identified in (Hermann and Heckert, 2005) at 70% sequence identity over 100bp or more between the rat and human loci.

^#Windowed-average sequence identity score between human, rat, and chicken Fshr(≥80%;≥100bp).

^§Relative to the Fshr transcriptional start sites.

^†The recognition sequence for EcoRV (5’-GCGCGATATC-3’) was present on the 5’end of each oligodeoxynucleotide (not shown).