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Schematic representation of cloning of siRNA-Hsp25. pSuper vector was used to introduce a 64-base oligomer to form the hairpin with a Hsp25-specific sequence. This 21-base was synthesized with defined sequences to provide two restriction sites (Bgl II and Hin d III) and, on transcription, form a hairpin, in order to initiate gene silencing. Eight clones were screened and three positive clones were sequenced. All experiments were performed with one of the matched sequences.
