Fig. 2.
Effect of transfection of siRNA-Hsp25 on 4T1 breast adenocarcinoma cells. 4T1 cells were transfected with GFPplasmid plus siRNA-Hsp25 plasmid as described in ‘Materials and Methods'. After 48 h, cells were sorted using flow cytometry for either GFP-negative or GFP-positive cells. Sorted cells were stained with anti-Hsp25 antibody and a secondary isotype-matched antibody biotinylated with Texas red. Cells were counterstained for nuclei integrity with DAPI (blue fluorescence). Immunofluorescent pictograms show GFP-negative cells express Hsp25 (red fluorescence) and GFP-positive cells are negative for Hsp25. Data are representative of at least three independently performed experiments with similar results.