Fig. 6.

Effect of siRNA-Hsp25 on cell cycle distribution. Freshly sorted 4T1 cells (10⁵) transfected with GFP^plasmid (a), siRNA-Hsp25 (b), pLXSN (c) or Hsp25^Neo (d) were fixed in ethanol and incubated with propidium iodide and RNAse A for 1 h at 37 °C. DNA content and cell cycle were performed using flow cytometric analysis as described in detail in ‘Materials and Methods'. Results are a representative of four independently performed experiments with similar results. Abscissa, fluorescence intensity; ordinate, relative cell number.