Fig. 7.

Suppression of migration of 4T1 cells upon transfection with siRNA-Hsp25. a 4T1 (10⁵) cells transfected with siRNA-Hsp25, GFP^plasmid or Hsp25^Neo were placed on Matrigel invasion chambers (BD Pharmingen). Twenty-two hours later, cells were harvested and stained with 0.5% crystal violet solution and mounted in a microscope slide at 10 × magnification. Data are representative of three independently performed experiments with similar results. b 4T1 (10⁵) cells transfected with siRNA-Hsp25, GFP^plasmid or Hsp25^Neo were placed on Matrigel invasion chambers (BD Pharmingen). Twenty-two hours later, cells were harvested and stained with 0.5% crystal violet solution and mounted in a microscope slide under 10 × magnification and 15 fields were counted. Bars are the mean percent migration ± SD and represent the sum of three independently performed experiments. ^* p < 0.05 versus control (Student’s t test).