. Author manuscript; available in PMC: 2007 Jan 5.

Published in final edited form as: Tumour Biol. 2005 Dec 8;27(1):17–26. doi: 10.1159/000090152

Table 1.

Effect of siRNA-Hsp25 on cell cycle distribution

4T1 transfection¹	Cells in respective phases of cell cycle², %
	Sub-G0/G1	G0/G1	S	G2/M
None	2.4±3	63.5±5	17.1±2	17.8±2
GFP^plasmid	1.0±1	58.5±4	20.6±2	13.2±1
siRNA-Hsp25	2.8±2	29.6±3	21.6±1	47.2±2
pLXSN	3.4±2	50.3±4	22.8±2	21.6±2
Hsp25^Neo	5.6±2	56.5±5	20.6±2	19.3±2

Results are expressed as mean ± SD.

Freshly sorted 4T1 cells (10⁵) were transfected with GFP^plasmid, siRNA-Hsp25, pLXSN or Hsp25^Neo and were fixed in ethanol and incubated with propidium iodide and RNAse A for 1 h at 37 ° C as described in detail in ‘Materials and Methods’.

Cell cycle analysis was performed using flow cytometric analysis as described in detail in ‘Materials and Methods’. Results are the percentage of cells in respective phases of cell cycle ± SD and are the sum of two to three independently performed experiments with similar results.