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Polarity of the oocyte microtubule cytoskeleton in Khc null oocytes. The localization of MTOC material was tested with antibodies to Centrosomin. (A) Wild-type and (B) Khc27/Khc27 stage 10A oocytes. The orientation of microtubule plus ends was tested by localizing a KHC::β-Gal fusion protein. (C) Wild-type and (D) Khc27/Khc27 late stage 9 egg chambers. The wild-type genotype included two copies of the fusion gene, whereas the mutant included one copy.
