TABLE 1.
Primers used for RT-PCR and qPCR
| Gene | Primer sequencea | Referenceb |
|---|---|---|
| ACE |
F: ATGTACCTGGAACCACAGCAG
R: GCTTGGGTATGTTCCACTCAA |
U03734 |
| Adiponectin |
F: CTCCACCCAAGGAAACTTGT
R: CTGGTCCACATTTTTTTCCT |
(Tsuda et al., 2004) |
| Angiotensinogen |
F: TTGTTGAGAGCTTGGGTCCCTTCA
R: CAGACACTGAGGTGCTGTTGTCCA |
(Shyu et al., 1995) |
| aP2 |
F: AGCGTAGAAGGGGACTTGGT
R: ATGGTGGTCGACTTTCCATC |
U75581 |
| AT1 |
F: GCACAATCGCCATAATTATCC
R: CACCTATGTAAGATCGCTTC |
(Llorens-Cortes et al., 1994) |
| AT2 |
F: TTATGGCTTTCCCACCTGAG
R: AAGGAAGTGCCAGGTCAATG |
U01908 |
| FAS |
F: GAGTCTGTCTCCCGCTTGAC
R: TGGAAATGAGGGCCATAGTC |
NM 017332 |
| GAPDH |
F: GTCGGTGTCAACGGATTTG
R: GGGTTTCCCATTGATGACC |
NM 017008 |
| GAPDH (qPCR) |
F: ATGACTCTACCCACGGCAAG
R: TGGAAGATGGTGATGGGTTT |
NM 017008 |
| GLUT4 |
F: TTTCCAGTATGTTGCGGATG
R: TCAGTCATTCTCATCTGGCC |
(Hosaka et al., 1992) |
| HSL |
F: ATCATTCCCAAGCCACAAAG
R: GTCCCCACGTGTTCATCTCT |
NM 012859 |
| Leptin |
F: CCAGGATGACACCAAAACCCTC
R: ATCCAGGCTCTCTGGCTTCTGC |
NM 013076 |
| Pref-1 |
F: ATCGTGACCAACAGCTGTACC
R: CTTTCATGGACACCTTCAGGA |
U25680 |
| PPAR γ |
F: CATTTCTGCTCCACACTATGAA
R: CGGGAAGGACTTTATGTATGAG |
(Tsuda et al., 2004) |
| Renin |
F: CTTGTTGCTCTGGACCTCTTG
R: TGACTTTGAAGGTCTGGGATG |
J02941 |
| Renin (qPCR) |
F: CTGTGCATACTGGCTCTCCA
R: GAACCCGATGCGATTGTTAT |
J022941 |
| Renin receptor (qPCR) |
F: TGGCCTATACCAGGAGATCG
R: AATAGGTTGCCCACAGCAAG |
AB188298 |
| TNF α |
F: CCACCACGCTCTTCTGTCTAC
R: ACCACCAGTTGGTTGTCTTTG |
X66539 |
a
F: Forward primer 5′-3′, R: Reverse primer 5′-3′
b
Reference shows GenBank accession number for primers designed in our laboratory or reference to literature for previously published primer sequences