Table 1.
cDNA sequence variation in circularized Lithobius mitochondrial tRNAs
| tRNA | No. clones | cDNA sequence |
|---|---|---|
| tRNA (C) | 7 | TCAGTCTACGACATCTTGAACCATCAAGATT |
| 1 | TCAGT–––tatcacacagcagtg–––AGATT | |
| 1 | TCAG–––––––––actag–––––––––GATT | |
| tRNA (E) | 10 | TGTTAAAAACTAGAAAGACCACTTTCTATAGTGAAA |
| 1 | TGTTA––––––AGAAAGACCACTTTCTATAGTGAAA | |
| tRNA (H) | 4 | TAACTTTTACCTACCAAGGTAAAATAGTTTAAGAAG |
| 2 | TAACTTTTACC-ACCA––––––––––––––––GAAG | |
| 6 | TAACTTTTGA–––––––GGTAAAATAGTTTAAGAAG | |
| 1 | TAACTTTTGAT––––––GATAAAATAGTTTAAGAAG | |
| tRNA (M) | 2 | TCTCTTACATACCAATGTAAGATAAGCTA |
| 4 | TCTCTTACA-ACCA-TGTAAGATAAGCTA | |
| 3 | TCTCTTCTTTTT–––TGTAAGATAAGCTA | |
| tRNA (N) | 4 | TAAGTTTTGACTTTACCAAAAGTCATGAAC |
| 1 | TAAGTTTT––––––––––AAAGTCATGAAC | |
| 1 | TAAGTTTTGACTTTACCAAAAGTCATGAAC | |
| 1 | TAAGTTTTGACTT-ACCA-AAGTCATGAAC | |
| 1 | TAAGTTTTGACTCTACCA-AAGTCATGAAC | |
| 4 | TAAGTTTTGAA–TGCTGCAAAGTCATGAAC | |
| 1 | TAAGTTTTGAAAATGCTGCAAAGTCATGAAC | |
| tRNA (Q) | 6 | AGTCTGTTAATGTAAACCATTACATTAGATG |
| 1 | AGTCTGTTAATGTAAAC––TTACATTAGATG | |
| 1 | AGTCTGTTA–––––tttt––––––TTAGATG | |
| tRNA (R) | 4 | GGGCACCCCCTCATAGACCACTATGAGADGCGA |
| 2 | GGGCACCCCCCTAAA––––––––––––AAGCGA | |
| 2 | GGGCACCAAAAA–––––––––––––––AAGCGA | |
| 1 | GGGCACCCCCAAAA–––––––––––––AAGCGA | |
| 1 | GGGCACCCCC––––––––––––––––––AGCGA | |
| tRNA (S1) | 12 | CACTTGTTATTATCTACCAAGATAATCGCG |
Underlined nucleotides are part of the primer sequence. Nucleotides in boldface are those inferred to be edited at the 3′end of the acceptor stem. Nucleotides in italics are inferred to be added by some alternative (nontemplated) editing process(es). Nucleotides in lowercase are inferred to be artifacts of the experimental procedure. D indicates nucleotide variation (A, T, or G) among the four edited cDNA clones for tRNA (R), possibly caused by posttranscriptional modification at this tRNA position (26).