Skip to main content
. Author manuscript; available in PMC: 2007 Jan 8.
Published in final edited form as: Poult Sci. 2006 Feb;85(2):278–287. doi: 10.1093/ps/85.2.278

Table 1.

Kinetic parameters using trans-1,3-diphenylpropene oxide (t-DPPO) as a substrate

Structure of t-DPPO Kinetic parameter Recombinant chicken sEH1 Recombinant mouse sEH Recombinant human sEH
graphic file with name nihms9498t1.jpg Specific activity (nmol/min per mg) 823.1 ± 27.3 17,000 ± 300.0 4,500 ± 200.0
Km (μM) 25.3 ± 0.9 4.3 ± 0.6 6.2 ± 0.6
kcat (/s) 0.9 ± 0.03 18.0 ± 0.3 4.3 ± 0.3
kcat/Km(μM/s) 0.04 4.2 0.7
1

Recombinant chicken soluble epoxide hydrolase (sEH) was partially purified as described. Assay conditions are described in the Materials and Methods section. Results are presented as the means ± standard deviations of 3 separate experiments. Values for the human and mouse enzyme are from Morisseau et al. (2000).