Table 2.
Specific activity (nmol/min per mg)
|
|||
---|---|---|---|
Compound name | Structure | Recombinant chicken sEH1 | Recombinant mouse* and mouse |
cis-9,10-epoxystearic acid | 3.1 ± 0.2 | 1,139 ± 34* | |
14,15-EET | 24.5 ± 2.1 | 1,260.0 | |
11,12-EET | 12.0 ± 1.2 | 640.0 | |
8,9-EET | 11.6 ± 0.1 | 370.0 |
Recombinant chicken soluble epoxide hydrolase (sEH) was partially purified as described. Assay conditions are described in the Materials and Methods section. Results are presented as the means ± standard deviations of 3 separate experiments. Values for the mouse enzyme assayed with cis-9,10-epoxystearic are from Morisseau et al. (2000). Values for the mouse enzyme assayed with the EET (epoxyeicosatrienoic acid) are from Chacos et al. (1983).