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. Author manuscript; available in PMC: 2007 Jan 8.
Published in final edited form as: Endocrinology. 2006 Jun 22;147(9):4330–4338. doi: 10.1210/en.2006-0120

Fig. 4.

Fig. 4

Chronic ethanol (EtOH) feeding did not increase the quantity of immunoreactive PDE4A, PDE4B, or PDE4D isoforms. Adipocytes isolated from pair- and ethanol-fed rats were homogenized in radio-immunoprecipitation buffer. After removal of the fat layer by centrifugation at 4000 × g for 3 min, the fluid fraction was prepared in SDS sample buffer and applied to a 6% SDS-polyacrylamide gel. PDE4 was immunoblotted using a PDE4 antibody (detecting all known PDE4 A and D variants) (A) and a PDE4B antibody (detecting all known PDE4B variants) (B). Rat testes were used as positive controls. A representative immunoblot from six experiments is shown. Asterisks indicate the immunoreactive PDE4 isoforms detected in rat testes, and the arrowheads indicate the corresponding bands detected in rat adipocytes.