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. Author manuscript; available in PMC: 2007 Jan 8.
Published in final edited form as: Endocrinology. 2006 Jun 22;147(9):4330–4338. doi: 10.1210/en.2006-0120

Fig. 6.

Fig. 6

Phosphorylation of perilipin A and HSL were detected by a phospho-(Ser/Thr) PKA substrate antibody. Adipocytes isolated from pair-fed rats were treated with or without 1 μm isoproterenol for 5 or 10 min, and fat layer-associated protein samples or whole cell homogenates were prepared as indicated. A, Fat layer-associated protein samples were subjected to SDS-PAGE and immunoblotted with antibodies against perilipin A/B (Peri A/B) or phospho-(Ser/Thr) PKA substrate (p-Ser/Thr). B, Cell homogenates were subjected to SDS-PAGE and immunoblotted with phospho-(Ser/Thr) PKA substrate antibody.