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. Author manuscript; available in PMC: 2007 Jan 8.
Published in final edited form as: Endocrinology. 2006 Jun 22;147(9):4330–4338. doi: 10.1210/en.2006-0120

Fig. 7.

Fig. 7

Chronic ethanol feeding reduced β-adrenergic receptor-stimulated phosphorylation of perilipin A and HSL. Adipocytes isolated from pair- and ethanol-fed rats were treated with or without 1 μm isoproterenol for 5 or 10 min, and fat layer-associated protein samples or whole cell homogenates were prepared as indicated. Phospho-perilipin A (p-Peri A; panel A) and phospho-HSL (p-HSL; panel B) were immunoblotted using a phospho-(Ser/Thr) PKA substrate antibody. A, A representative immunoblot of phospho-perilipin A is shown; the image shown for homogenate is from a longer exposure compared with the fat layer. With equal exposure times, phospho-perilipin A was 3.8 arbitrary units of density in fat layer compared with 1 in homogenate. B, A representative immunoblot for phospho-HSL is shown with equal exposure times for the fat layer and homogenate. Caveolin was used as a control for equal protein extraction from fat samples; ERK1 was used as a loading control in the cell homogenates. Values represent means ±sem (n = 4–6). *, P < 0.05, pair-fed vs. EtOH-fed. PF, Pair-fed; EF, ethanol-fed.