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. Author manuscript; available in PMC: 2007 Jan 8.
Published in final edited form as: Endocrinology. 2006 Jun 22;147(9):4330–4338. doi: 10.1210/en.2006-0120

Fig. 8.

Fig. 8

Chronic ethanol feeding did not affect dibutyryl-cAMP-stimulated phosphorylation of perilipin A or HSL. Adipocytes isolated from pair- and ethanol-fed rats were treated with or without 0.5 mm dibutyryl-cAMP (db-cAMP) for 5 or 10 min, and fat layer-associated protein samples or whole cell homogenates were prepared as indicated. Phospho-perilipin A (p-Peri A; panel A) and phospho-HSL (pHSL; panel B) were immunoblotted using a phospho-(Ser/Thr) PKA substrate antibody. A representative immunoblot from four experiments is shown. PF, Pair-fed; EF, ethanol-fed.