Figure 3.

A W286C mutant or G288SΔC5 deletion of M. tuberculosis LeuRS abolishes complementation. Mutations were introduced into M. tuberculosis LeuRS genes using pC³-Mtb and tested for complementation of null strain QBY320. The G288S mutant and wild-type M. tuberculosis gene complemented the null strain. Similar to previous studies using yeast mitochondrial LeuRS (11), a W286C and a G288SΔC5 mutation in the M. tuberculosis protein did not complement strain QBY320 on glycerol medium. A vector only control (pQB153) also was included. All strains grew on medium containing glucose that does not require mitochondrial function.