Skip to main content
. Author manuscript; available in PMC: 2007 Jan 8.
Published in final edited form as: Biochemistry. 2006 Dec 26;45(51):15670–15683. doi: 10.1021/bi061718f

Figure 10.

Figure 10

Inhibition of myosin motor decreases h2-calponin gene expression in NIH 3T3 cells. NIH 3T3 mouse fibroblasts cultured on plastic substrate were treated with 100 μM blebbistatin for 3 days. The control cells were treated with 0.2% DMSO solvent control. (A) Phase contrast images show the slack morphology of the blebbistatin-treated cells. (B) Northern blotting was carried out with 32P-labeled h2-calponin specific cDNA probe on total RNA extracted from the cells. Normalized by the ribosomal RNA levels, the autoradiograph shows decreased expression of h2-calponin mRNA in the blebbistatin-treated cells. (C) The corresponding decrease in h2-calponin protein is demonstrated by Western blot using the anti-h2-calponin antibody RAH2.