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. Author manuscript; available in PMC: 2007 Jan 8.

Published in final edited form as: Biochemistry. 2006 Dec 26;45(51):15670–15683. doi: 10.1021/bi061718f

Cyclic stretching decreases h2-calponin expression in alveolar cells. (A) SW1573 human alveolar cells were cultured on plastic dish or rubber membranes without stretching for 3 days. Total protein extracts were examined by SDS-PAGE and RAH2 antibody Western blot. The SDS-gel and Western blots densitometry quantifications against the level of actin show that alveolar cells statically cultured on plastic dish and rubber membrane had similar protein contents and high h2-calponin expression, indicating that they both provide cultural substrates non-deformable by the force generated in alveolar cells. (B) RLE-6TN rat alveolar cells cultured on rubber membranes were treated with cyclic stretching for 3 days. Total protein extracts were examined as above and SDS-gel and Western blots densitometry quantifications against the level of actin, total cellular protein, or histones showed that cyclic stretch significantly decreased the level of h2-calponin expression in the cells (*P<0.005, **P<0.001). The results are summarized from 6 individual experiments.

Cyclic stretching decreases h2-calponin expression in alveolar cells. (A) SW1573 human alveolar cells were cultured on plastic dish or rubber membranes without stretching for 3 days. Total protein extracts were examined by SDS-PAGE and RAH2 antibody Western blot. The SDS-gel and Western blots densitometry quantifications against the level of actin show that alveolar cells statically cultured on plastic dish and rubber membrane had similar protein contents and high h2-calponin expression, indicating that they both provide cultural substrates non-deformable by the force generated in alveolar cells. (B) RLE-6TN rat alveolar cells cultured on rubber membranes were treated with cyclic stretching for 3 days. Total protein extracts were examined as above and SDS-gel and Western blots densitometry quantifications against the level of actin, total cellular protein, or histones showed that cyclic stretch significantly decreased the level of h2-calponin expression in the cells (*P<0.005, **P<0.001). The results are summarized from 6 individual experiments.