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. 2007 Jan 17;2(1):e159. doi: 10.1371/journal.pone.0000159

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Williams et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Representative Western blots showing NFkB, c-Fos and c-Jun and pSTAT 3 levels in response to CD154/C4BP stimulation. Aliquots of nuclear or cytoplasmic extracts as appropriate (40 ug protein) from cultured and stimulated primary human cholangiocytes were probed for NFkB (panel I); c-Fos and c-Jun(panel II) or pSTAT3 content. Blots were stripped and re-probed for beta-actin which allowed for normalisation of data for variations in protein loading.