Table 1.
Strain names, strain constructions, and relevant genotypes
| Strain name | Strain construction or reference | Relevant genotype* |
|---|---|---|
| W303a | Ref. 23 | Wild-type |
| W1588-4c | Provided by R. Rothstein | RAD5 |
| JMY48 | Transformation of W303a with plasmid pKR5 | Wild-type, plasmid-borne TEL1-HA |
| JMY73 | Ref. 3 | sml1∷HIS3 |
| KRY22 | Transformation of W303a with PCR fragment to introduce HA tag into TEL1† | TEL1-HA |
| MD78 | Transformation of JMY73 with PCR fragment to introduce HA tag into MEC1† | sml1∷HIS3 MEC1-HA |
| MD85 | Two-step transplacement of MD78 with pMD81† | sml1∷HIS3 mec1-HA(KD) |
| JMY300-1a | Ref. 3 | α tel1∷ura3 mec1-21 |
| RCY278-5b | Provided by R. Craven | α RAD5‡ |
| JMY303 | Ref. 3 | a/α tel1∷ura3/TEL1 mec1-21/MEC1 |
| sml1∷HIS3/SML1 | ||
| JMY303-2a | Spore of JMY303 | tel1∷ura3 |
| KRY20a | Ref. 3 | ura3Δ∷HIS3 tel1∷ura3 ade3 |
| JMY103 | Transformation of KRY20a with plasmid pJM8 | tel1∷ura3, plasmid-borne tel1-HA(KD) |
| JMY303-1c | Spore of JMY303 | α mec1-21 |
| JMY303-3a | Spore of JMY303 | α sml1∷HIS3 tel1∷ura3 |
| JMY303-8a | Spore of JMY303 | α tel1∷ura3 |
| JMY303-8d | Spore of JMY303 | mec1-21 sml1∷HIS3 |
| JMY312 | Cross of MD78 and JMY303-8a | a/α tel1∷ura3/TEL1 sml1∷HIS3/SML1 |
| MEC1-HA/MEC1 | ||
| JMY312-6d | Spore derived from JMY312 | sml∷HIS3 tel1∷ura3 MEC1-HA |
| JMY312-8c | Spore derived from JMY312 | sml∷HIS3 tel1∷ura3 MEC1-HA |
| JMY313 | Cross of MD85 and JMY303-3a | a/α mec1-HA(KD)/MEC1 tel1∷ura3/TEL1 |
| sml1∷HIS3/sml1∷HIS3 | ||
| JMY313-9a | Spore derived from JMY313 | mec1-HA(KD) sml1∷HIS3 tel1∷ura3 |
| JMY314 | Cross of RCY278-5b and MD85 | a/α mec1-HA(KD)/MEC1 rad5-535/RAD5 |
| sml1∷HIS3/SML1§ | ||
| JMY314-3d | Spore derived from JMY314 | mec1-HA(KD) sml1∷HIS3 rad5-535 |
| JMY314-5a | Spore derived from JMY314 | α mec1-HA(KD) sml1∷HIS3 RAD5 |
*
Most of the strains in the study are isogenic (except for changes introduced by transformation) with W303a (a leu2-3,112 his3-11,15 ura3-1 ade2-1 trp1-1 rad5-535 can1-100). All genes that differ from those of the progenitor genotype are listed.
†
Details of these constructions are in Materials and Methods.
‡
This strain also contains an insertion of URA3 near the telomere of chromosome XVL (TELXVL∷URA3; [24]).
§
This diploid is heterozygous for the TELXVL∷URA3 marker.