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(A) Transfection of indicated amounts of Tat- or TatF38A-expressing constructs in Jurkat 1G5 cells using the DEAE-dextran method. (B) Cotransfection of indicated amounts of Tat or TatF38A with the HIV LTR luciferase (200 ng) and wild type or kinase-deficient RSK2 (200 ng) in CLS cells. Values are means±SEM of five experiments for wild type and three experiments for mutant Tat.
