Feeding increases NAPE-PLD activity and expressions in rat jejunum. Effects of free feeding (FF), 24-h food deprivation (FD), and refeeding (10, 30, 60 and 120 min) on NAPE-PLD activity (A), NAPE-PLD mRNA expression (B) and NAPE-PLD protein levels (B inset). *p < 0.05, **p < 0.01 vs FD, n = 6. C, Representative immunostaining showing the localization of NAPE-PLD in the duodenal epithelium and lamina propria of wild-type C57/BL6 mice. D, Absence of immunoreactive NAPE-PLD in corresponding structures of NAPE-PLD−/− mice; Scale bar, 50 μm.