Pituitary-specific expression of mRNA for ovine FSHB in Tg(FSHB-rtTA, tetO-FSHB)1Wmil mice during Dox treatment. Male mice from Tg(FSHB-rtTA, tetO-FSHB)1Wmil founders were treated with Dox for two weeks, and then tissues were taken and assayed for mRNA of ovine FSHB using real-time RT-PCR. All samples from individual mice were assayed together and the results were normalized to pituitary expression of ‘‘100.’’ To determine differences between several means, analysis of variance was used, followed by the Tukey multiple comparison test for post hoc evaluation of differences between different treatment groups. Assay variation was ≤10%. Individual percentage expressions for brain, heart, lung, liver, spleen, and gonad were 0.9 ± 0.7, 1.5 ± 1.2, 0.7 ± 0.7, 1.0 ± 0.7, 1.9 ± 0.6, and 0.9 ± 0.4, respectively, compared to expression of mRNA for FSHB in the pituitary.