FIG. 5.

Dox increased total FSH and mRNA for FSHB by 50%–60% in male Tg(FSHB-rtTA, tetO-FSHB)1Wmil mice. No increase was observed in males lacking the fertility switch (not shown). Transgenic B6SJL founder mice were bred into CD-1 mice, giving them considerable CD-1 genetic makeup. Data from the fourth generation are presented in this report. Males were fed rodent chow ± 6g/kg of Dox for 14 days, and then their pituitaries and blood were analyzed. Total RNA was isolated, and mRNAs for ovine FSHB and mouse FSHB were quantified in 100 ηg of RNA using real-time RT-PCR. Standard curves for PCR analyses consisted of known amounts of cDNA for ovine or mouse FSHB in plasmids. Each data point represents the mean ± SEM for either four hemizygous or seven homozygous mice. Statistical analysis used analysis of variance plus the Tukey multiple comparison test. There are significant differences (P < 0.05) between means with different letters.