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. 1986 Oct;30(4):584–589. doi: 10.1128/aac.30.4.584

Effect of clavulanic acid on the activity of ticarcillin against Pseudomonas aeruginosa.

F Tausk, C W Stratton
PMCID: PMC176485  PMID: 3098162

Abstract

We studied the ability of clavulanic acid (CA) to induce beta-lactamase in Pseudomonas aeruginosa isolates and what effect this might have on the susceptibilities to beta-lactam agents. We first used a disk approximation method to test 4 laboratory and 16 clinical P. aeruginosa isolates against antipseudomonal beta-lactam agents for truncation by CA and found this to be very common. All antimicrobial compounds except imipenem demonstrated truncation in the vicinity of CA. We also evaluated the extent to which chromosomal beta-lactamase is induced by CA and found this to occur to some degree in most isolates and to be dependent on the concentration of CA. Finally, we performed time kill curves on these isolates to compare bacterial growth in ticarcillin alone with growth in ticarcillin-CA (the CA at 2 or 4 micrograms/ml). We found that CA at this concentration has neither an antagonistic nor a synergistic antibacterial effect in combination with ticarcillin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett S., Wise R., Weston D., Dent J. Pharmacokinetics and tissue penetration of ticarcillin combined with clavulanic acid. Antimicrob Agents Chemother. 1983 Jun;23(6):831–834. doi: 10.1128/aac.23.6.831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bolivar R., Weaver S. S., Bodey G. P. Activity of beta-lactamase inhibitors in combination with new beta-lactam antibiotics against resistant gram-negative organisms. Diagn Microbiol Infect Dis. 1984 Jun;2(3):255–260. doi: 10.1016/0732-8893(84)90039-7. [DOI] [PubMed] [Google Scholar]
  3. Brittain D. C., Scully B. E., Neu H. C. Ticarcillin plus clavulanic acid in the treatment of pneumonia and other serious infections. Am J Med. 1985 Nov 29;79(5B):81–83. doi: 10.1016/0002-9343(85)90134-2. [DOI] [PubMed] [Google Scholar]
  4. Calderwood S. B., Gardella A., Philippon A. M., Jacoby G. A., Moellering R. C., Jr Effects of azlocillin in combination with clavulanic acid, sulbactam, and N-formimidoyl thienamycin against beta-lactamase-producing, carbenicillin-resistant Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1982 Aug;22(2):266–271. doi: 10.1128/aac.22.2.266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chalkley L. J., Koornhof H. J. Antimicrobial activity of ciprofloxacin against Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus determined by the killing curve method: antibiotic comparisons and synergistic interactions. Antimicrob Agents Chemother. 1985 Aug;28(2):331–342. doi: 10.1128/aac.28.2.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. File T. M., Jr, Tan J. S., Salstrom S. J., Johnson L. A., Douglas G. F. Timentin versus piperacillin or moxalactam in the therapy of acute bacterial infections. Antimicrob Agents Chemother. 1984 Sep;26(3):310–313. doi: 10.1128/aac.26.3.310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fuchs P. C., Barry A. L., Thornsberry C., Jones R. N. In vitro activity of ticarcillin plus clavulanic acid against 632 clinical isolates. Antimicrob Agents Chemother. 1984 Mar;25(3):392–394. doi: 10.1128/aac.25.3.392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gootz T. D., Sanders C. C. Characterization of beta-lactamase induction in Enterobacter cloacae. Antimicrob Agents Chemother. 1983 Jan;23(1):91–97. doi: 10.1128/aac.23.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hallander H. O., Dornbusch K., Gezelius L., Jacobson K., Karlsson I. Synergism between aminoglycosides and cephalosporins with antipseudomonal activity: interaction index and killing curve method. Antimicrob Agents Chemother. 1982 Nov;22(5):743–752. doi: 10.1128/aac.22.5.743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hunter P. A., Coleman K., Fisher J., Taylor D. In vitro synergistic properties of clavulanic acid, with ampicillin, amoxycillin and ticarcillin. J Antimicrob Chemother. 1980 Jul;6(4):455–470. doi: 10.1093/jac/6.4.455. [DOI] [PubMed] [Google Scholar]
  11. Johnson C. C., Reinhardt J. F., Wallace S. L., Terpenning M. S., Helsel C. L., Mulligan M. E., Finegold S. M., George W. L. Safety and efficacy of ticarcillin plus clavulanic acid in the treatment of infections of soft tissue, bone, and joint. Am J Med. 1985 Nov 29;79(5B):136–140. doi: 10.1016/0002-9343(85)90147-0. [DOI] [PubMed] [Google Scholar]
  12. Livermore D. M. Kinetics and significance of the activity of the Sabath and Abrahams' beta-lactamase of Pseudomonas aeruginosa against cefotaxime and cefsulodin. J Antimicrob Chemother. 1983 Feb;11(2):169–179. doi: 10.1093/jac/11.2.169. [DOI] [PubMed] [Google Scholar]
  13. Minami S., Yotsuji A., Inoue M., Mitsuhashi S. Induction of beta-lactamase by various beta-lactam antibiotics in Enterobacter cloacae. Antimicrob Agents Chemother. 1980 Sep;18(3):382–385. doi: 10.1128/aac.18.3.382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Neu H. C., Fu K. P. Clavulanic acid, a novel inhibitor of beta-lactamases. Antimicrob Agents Chemother. 1978 Nov;14(5):650–655. doi: 10.1128/aac.14.5.650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Neu H. C., Fu K. P. Synergistic activity of piperacillin in combination with beta-lactamase inhibitors. Antimicrob Agents Chemother. 1980 Oct;18(4):582–585. doi: 10.1128/aac.18.4.582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Paisley J. W., Washington J. A., 2nd Combined activitiy of clavulanic acid and ticarcillin against ticarcillin-resistant, gram-negative bacilli. Antimicrob Agents Chemother. 1978 Aug;14(2):224–227. doi: 10.1128/aac.14.2.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Roselle G. A., Bode R., Hamilton B., Bibler M., Sullivan R., Douce R., Staneck J. L., Bullock W. E. Clinical trial of the efficacy and safety of ticarcillin and clavulanic acid. Antimicrob Agents Chemother. 1985 Mar;27(3):291–296. doi: 10.1128/aac.27.3.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sanders C. C., Sanders W. E., Jr Emergence of resistance during therapy with the newer beta-lactam antibiotics: role of inducible beta-lactamases and implications for the future. Rev Infect Dis. 1983 Jul-Aug;5(4):639–648. doi: 10.1093/clinids/5.4.639. [DOI] [PubMed] [Google Scholar]
  19. Scully B. E., Chin N. X., Neu H. C. Pharmacology of ticarcillin combined with clavulanic acid in humans. Am J Med. 1985 Nov 29;79(5B):39–43. doi: 10.1016/0002-9343(85)90127-5. [DOI] [PubMed] [Google Scholar]
  20. Stratton C. W. Susceptibility testing revisited. Prog Clin Pathol. 1984;9:65–100. [PubMed] [Google Scholar]
  21. Tausk F., Evans M. E., Patterson L. S., Federspiel C. F., Stratton C. W. Imipenem-induced resistance to antipseudomonal beta-lactams in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1985 Jul;28(1):41–45. doi: 10.1128/aac.28.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Vu H., Nikaido H. Role of beta-lactam hydrolysis in the mechanism of resistance of a beta-lactamase-constitutive Enterobacter cloacae strain to expanded-spectrum beta-lactams. Antimicrob Agents Chemother. 1985 Mar;27(3):393–398. doi: 10.1128/aac.27.3.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. White A. J., Curtis N. A. Hydrolysis of beta-lactamase-stable beta-lactams by type I 'sponge' beta-lactamases. J Antimicrob Chemother. 1985 Sep;16(3):403–405. doi: 10.1093/jac/16.3.403. [DOI] [PubMed] [Google Scholar]
  24. Williams M. E., Harman C., Scheld M., Hess C. E., Donowitz G. R. A controlled study of ticarcillin plus clavulanic acid versus piperacillin as empiric therapy for fever in the immunocompromised host. Am J Med. 1985 Nov 29;79(5B):67–72. doi: 10.1016/0002-9343(85)90131-7. [DOI] [PubMed] [Google Scholar]
  25. Wise R., Andrews J. M., Bedford K. A. In vitro study of clavulanic acid in combination with penicillin, amoxycillin, and carbenicillin. Antimicrob Agents Chemother. 1978 Mar;13(3):389–393. doi: 10.1128/aac.13.3.389. [DOI] [PMC free article] [PubMed] [Google Scholar]

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