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. 2007 Jan 11;117(2):407–418. doi: 10.1172/JCI29539

Figure 4. WASp is not required for generation of Tregs within the thymus.

Figure 4

(A) WASp is not required for the production of peripheral Tregs. Peripheral LN cells from WT or WASp–/– animals were stained simultaneously for CD4, CD25, and Foxp3 and evaluated by flow cytometry. Note that Foxp3+ Tregs are present in WASp–/– mice albeit at a slightly decreased percentage. (B) WASp–/– and WT mice have a similar percentage of Tregs (CD4+ CD25+Foxp3+) within the CD4+ SP thymic population. Both 6- and 16-week-old WT and WASp–/– C57BL/6 mice (n = 5 for each age and strain) were evaluated. (C) The selective advantage of WASp+ T cells is not manifest in the thymus. The percentage of WASp+ cells was evaluated within various thymic cell subsets in 6- to 8-week-old WASp+/– heterozygous female carriers (C57BL/6 strain) (n = 5). Error bars show SD. Relative WASp expression was not significantly different among any subset evaluated. DN, CD4–CD8– thymocytes. Representative data from 1 of at least 3 experiments are shown.