Table 5.

Matrix for hybrid design with the experimental and predicted values of P450 BM-3 activity

Run	FeCl3	Induction at OD578	Induction time	Inoculum concentration	Observed response (×10−3 U/ml)	Predicted response (×10−3 U/ml)
1	0	0	0	1.7654	56.44	56.44
2	−1	−1	−1	0.5675	33.33	34.37
3	1	−1	−1	0.5675	44.92	43.88
4	−1	1	−1	0.5675	41.93	40.89
5	1	1	−1	0.5675	41.29	42.33
6	−1	−1	1	0.5675	39.92	38.88
7	1	−1	1	0.5675	35.53	36.57
8	−1	1	1	0.5675	48.79	49.83
9	1	1	1	0.5675	40.49	39.45
10	1.4697	0	0	−1.0509	46.29	46.29
11	−1.4697	0	0	−1.0509	40.27	40.27
12	0	1.4697	0	−1.0509	36.59	36.59
13	0	−1.4697	0	−1.0509	48.14	48.14
14	0	0	1.4697	−1.0509	59.39	59.39
15	0	0	−1.4697	−1.0509	50.45	50.45
16	0	0	0	0	57.20	57.20