Figure 4.

TLR6 cooperates with TLR2 in mediating proinflammatory signals. (A) The relative activity of an NF-κB luciferase reporter was assessed in CHO cells transiently expressing the indicated TLR constructs without further stimulation, or after stimulation by the indicated bacterial products. (B) RAW-TT10 macrophages were transfected with the indicated CD4-TLR chimeric constructs, and the production of TNF-α was measured by flow cytometry. The representative density plot shows that expression of CD4-TLR4 results in the production of TNF-α. Histograms were generated by using the gates shown. Thin lines indicate TNF-α production by macrophages that did not express the indicated CD4-TLRs (gate 1). Thick lines indicate TNF-α production by cells expressing CD4-TLRs (gate 2). The y axes of the histograms indicate relative cell number, and the x axes indicate TNF-α production on a log scale identical to that shown (on the y axis) in the density plot.