Effects of wild-type and 3A-2 mutant poliovirus infections on antigen
presentation to CTL. Chimpanzee target cells were infected with
wild-type vaccinia (wt-VV), rVV-NS3/4, wild-type (wt) poliovirus,
3A-2 mutant poliovirus, or coinfected with rVV-NS3/4 and rVV-GFP;
rVV-NS3/4 and rVV-3A,GFP; rVV-NS3/4 and wild-type poliovirus, or
rVV-NS3/4 and 3A-2 poliovirus. At 12 h after infection, target
cells were incubated for 4 h with T14 CTL at an effector/target
ratio of 1:1. Granzyme A secretion into the supernatants was determined
by enzymatic assay. Data are presented as the means of triplicate
samples, and the standard deviations are indicated.