Fig. 1.

Bcl10 regulates LPA-induced NF-κB activation. (A and B) Bcl10^+/− or Bcl10^−/− MEFs were preincubated with cycloheximide and then stimulated with 10 μM LPA (A) or 10 ng/ml TNF-α (B) for the indicated times. Cell extracts were separated on 10% polyacrylamide gels, subjected to immunoblotting with anti-IκB-α antibody, and reprobed with anti-β-actin antibody to show equal loading. Experiments were repeated five times. (C and D) Bcl10^+/− or Bcl10^−/− MEFs were stimulated with LPA (C) or TNF-α (D) as described above; nuclear extracts were prepared and incubated with radiolabeled oligonucleotides specific for NF-κB or NF-Y as a loading control. Results are representative of three independent experiments.