Fig. 2.

Retroviral reconstitution of Bcl10 gene-deficient MEFs rescues IκB-α degradation in response to LPA stimulation. (A) Bcl10-deficient MEFs were infected with the retroviral vector pBABE-puro encoding full-length Bcl10 (pBABE-puro-Bcl10) or an empty vector (pBABE-puro) as a negative control. Cell extracts were prepared and subjected to immunoblotting with anti-Bcl10-antibody and anti-β-actin antibody as a loading control. (B) Bcl10-deficient MEFs retrovirally infected with pBABE-puro or pBABE-puro-Bcl10 were stimulated with 10 μM LPA as described above, and cell extracts were subjected to immunoblotting by using antibodies against IκB-α and β-actin. Experiments were repeated three times.