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Normal LPA-induced MAPK and Akt activation in Bcl10-deficient MEFs. Bcl10+/− or Bcl10−/− MEFs were stimulated with 10 μM LPA as indicated. Cell extracts were separated on polyacrylamide gels and immunoblotted with antibodies against phospho-p38, phospho-p44/p42 (phospho-Erk), phospho-Jnk, or phospho-Akt. After stripping, membranes were reprobed with antibodies against p44/p42 (Erk) or β-actin to control for equal loading. Results are representative of five independent experiments.
